Journal: Liver Research

Article Title: Therapeutic potential of miRNA-26a-encapsulated nanoparticles against hepatocellular carcinoma in a murine model

doi: 10.1016/j.livres.2025.10.002

Figure Lengend Snippet: Relative quantification (RQ) values in the examined groups of : (A) P70S6K , (B) TGF-β, (C) DNMT3b, and (D) Caspase-3 genes . Abbreviations: HCC, hepatocellular carcinoma; NPs, nanoparticles; RQ, relative quantification. ∗significant difference ( P < 0.05). ∗∗significant difference ( P < 0.01). ∗∗∗significant difference ( P < 0.001). ns, not significant.

Article Snippet: The expression of 70 kDa ribosomal protein S6 kinase (p70S6K), transforming growth factor beta (TGF-β), DNA methyltransferase 3 beta (DNMT3b), and Caspase-3 genes was analyzed utilizing the TaqMan gene expression master mix (Thermo Fisher Scientific, Waltham, MA, USA) in accordance with the manufacturer’s instructions.

Techniques: Quantitative Proteomics

Journal: Frontiers in Immunology

Article Title: From DGCR8 expression analysis to diseased pathways in 22q11.2 deletion syndrome

doi: 10.3389/fimmu.2025.1611527

Figure Lengend Snippet: (A–D) Scatter plot showing the correlation between DGCR8 gene expression levels and NK cell, as percentage (A, patients; C, controls), and as cell count (B, patients; D, controls). The red dots indicate the patient without DGCR8 deletion. (E) Correlation between the activation of PI3K pathway assessed by measure of S6 phosphorylation, after PBMCs stimulation and absolute counts of B cells. S6 phosphorylation was not performed in patient without DGCR8 deletion. Abbreviations: pS6 (phosphorylated S6 protein); PMA-iono (phorbol-12-myristate-13-acetate and ionomycin).

Article Snippet: To assess the phosphorylation level of the ribosomal protein S6 (a marker of PI3K/Akt/mTOR pathway activation), PBMCs were stimulated with PMA-ionomycin (Cell Stimulation Cocktail 500X, eBioscience) or Dynabeads Human T-activator CD3/CD28 (Gibco by Life Technologies) for 3 hours at 37°C, 5% CO2.

Techniques: Gene Expression, Cell Counting, Activation Assay, Phospho-proteomics

Journal: Frontiers in Immunology

Article Title: From DGCR8 expression analysis to diseased pathways in 22q11.2 deletion syndrome

doi: 10.3389/fimmu.2025.1611527

Figure Lengend Snippet: (A) Scatter plot showing the percentage of cells phosphorylating S6 for two stimulation conditions: PMA-ionomycin and Dynabeads Human T-activator CD3/CD28. For each experimental condition, data are presented as the ratio of the percentage observed in the patient to that observed in the corresponding healthy donor. (B) Scatter plot illustrating the kinetics of γH2AX phosphorylation in patients versus controls, reflecting DNA damage response by assessing both the extent of damage and the efficiency of repair. γH2AX phosphorylation levels were measured at several time points post-bleomycin treatment: immediately after the 1-hour stimulation, and at 3- and 20-hours following drug removal. These data were used to calculate the Area Under the Curve (AUC), providing a comprehensive summary of the response dynamics. The AUC ratio (patient AUC divided by control AUC) is displayed for each case. (C) Scatter plot showing how a patient’s immune cells respond to stimulation compared to those of a healthy subject. Specifically, each point represents the ratio of phosphorylated STAT1 levels (measured by mean fluorescence intensity, MFI) in the patient’s cells to the corresponding levels in healthy control cells. The dashed line at a ratio of 1 indicates similar responses between patients and controls. pS6, phosphorylated S6 protein; PMA-iono, phorbol-12-myristate-13-acetate and ionomycin; AUC, Area Under the Curve; MFI, mean fluorescence intensity.

Article Snippet: To assess the phosphorylation level of the ribosomal protein S6 (a marker of PI3K/Akt/mTOR pathway activation), PBMCs were stimulated with PMA-ionomycin (Cell Stimulation Cocktail 500X, eBioscience) or Dynabeads Human T-activator CD3/CD28 (Gibco by Life Technologies) for 3 hours at 37°C, 5% CO2.

Techniques: Phospho-proteomics, Control, Fluorescence